Chromatogram
Neighbour-joining tree
Pairwise identity
Share of identical residues across columns where both sequences have dataSequence analysis
ORFs, G+C frame plot, restriction map, protein profiles, reverse translationVisualize
Look at your sequences, then trust them.
Open Sanger trace files, align reads to a reference, and walk every difference with the raw chromatogram right underneath.
Drop files here
.ab1 / .scf.fasta / .fastq.gb.aln.dnascale
1Open readsTrace files are trimmed by quality automatically and reverse reads are flipped to match.
2AlignMap reads to a reference, or build a multiple alignment of anything.
3VerifyJump from difference to difference with the chromatogram centred on each base.